Text

Laboratory Exercises

Biology 100A

HOME

On this site you will find

Background Information (Appendix)
Laboratory Exercises with Links

Please print the laboratory exercises before coming to lab, give yourself time to do the pre-lab exercises.

Background Information (Appendix)	Links
Laboratory Safety
Laboratory Notebook
Laboratory Reports	Author guidelines for PLoS submissions, includes how to reference citations.
Units and Measures
Extra Credit Service Project	Photos from Wolfback Ridge 4/18
What is Plagiarism?
SCANS Competencies including a list of competencies covered in this course	This is a useful summary of techniques covered in this course - use it when applying for a job or internship!

Day WF add 1 day	Laboratory Exercise Print before lab Do "Before lab" assignments in your notebook Bring to class	Links to required readings Read/watch before coming to class
1/17	Introduction to the class, Scientific method, Protocol writing - First day handout	Read: Laboratory Safety Read: Laboratory Notebook
1/19	Model cell I included in print package 1
1/24	Model cell II - included in print package 1 Metric conversion due on BioPortal
1/26	Data collection, organization, and visualization - included in print package 1 quiz due on BioPortal	Read: Laboratory reports Read: Plagiarism Author guidelines for PLoS submissions, includes how to reference citations.
1/31	Assay development - Chymosin I Pre-lab pipet and metric conversion quiz due on BioPortal	Read: (Micro)Pipettors (you need this information for the quiz) Do: Metric conversion tutorial Read: Units and Measures
2/2	Chymosin optimization Lab report 1 paper copy due in lab!	Grading rubric for lab report
2/7	Scientific literature I	Watch: Video of experiment (Science) Optional: Article summarizing the research report: Jailbreak rat (Scientific American)
2/9	Introduction to microscopy Pre-lab quiz on microscopes due on BioPortal	Read: Microscopy background
2/14	Mitosis	Watch: Mitosis animation
2/16	No lab
2/21	Meiosis, Chi square	Read: Chi square background
2/23	Amylase Pre-lab quiz on centrifuges due on BioPortal	Read: How City College and E. coli got their amylase - by Dr. Sheri Miraglia Read: Centrifuges (you need this information for the quiz)
2/28	Genetics problems Chi square
3/1	Lab midterm Notebook due
3/6	Aseptic techniques
3/8	ELISA
3/13	PCR I PCR II	Watch: PCR animation (you need this information for the quiz)
3/15	PCR II Report 2 due, upload to BioPortal Pre-lab quiz on PCR and electrophoresis due	Watch: Electrophoresis animation Read: Introduction to electrophoresis
3/20	Scientific literature II	Article: Quantifying the Integration of Quorum-Sensing Signals with Single-Cell Resolution Synopsis: Going With the Glow: How Bacteria Integrate Molecular Signals to Synchronize Bioluminescence TED talk by Bonnie Bassler (we will watch this together in lab):
3/22	no lab
4/3	Bioinformatics online lab, we will do this in class together Read up to "Questions to answer", answer discussion questions in your notebook.	Review: Bioinformatics Power Point Presentation adapted from Alexandra Schnoes
4/5	GE I
4/10	Bioethics Bioethics Presentation due	Optional: TED talk by Paul Root Wolfe on an astonishing series of recent bio-engineering experiments
4/12	GE II	Review: Introduction to electrophoresis
4/17	Diet analysis	Familiarize yourself with this online diet and exercise analysis tool
4/19	GE III Scientific literature 3, preparation Group role profiles	In a group of 3-4 students, choose one of the articles below. Note that often there are easier articles about the articles available. Do a search! Lots of students got interested in the bioethics discussion. You might want to find an article on the neurobiological underpinnings of morality! Chen et al. Common oxytocin receptor gene (OXTR) polymorphism and social support interact to reduce stress in humans. Cordey et al. Rhinovirus genome evolution during experimental human infection Soo et al. Malignancy without immortality? - Thanks, Quentin! Foerde et al. Modulation of competing memory systems by distraction Jin et al. Modeling Retinal Degeneration Using Patient-Specific Induced Pluripotent Stem Cells Scheeren et al. Antigen-Specific Monoclonal Antibodies Isolated from B Cells Expressing Constitutively Active STAT5 Epel et al. Accelerated telomere shortening in response to life stress Ophir et al. Cognitive control in media multitaskers Vlassenko et al. Spatial correlation between brain aerobic glycolysis and amyloid-! (A!) deposition Just added! Saayman et al. The Efficacy of Generating Three Independent Anti-HIV-1 siRNAs from a Single U6 RNA Pol III-Expressed Long Hairpin RNA Research article on autoinduction
4/24	Cardiovasculary System
4/26	No lab
5/1	Autoinduction check your results the next day In class we will watch an energetic, 18-minute TED talk on autoinduction by Bonnie Bassler	Watch: Lux operon animation If you loved this talk, you might want to check out Dr. Bassler's longer seminars (Bioseminar, HHMI Holiday Lecture)
5/3	Drugs and the brain
5/8	Lab review
5/10	Scientific Literature 3 - Student presentations Presentation rubric (adjust to 36 points total) + 4 for participation in discussion
5/15	Lab final
		GFP site by Mark Zimmer Video on the importance of GFP for biomecical research GFP: from jellyfish to the Nobel prize and beyond, Marc Zimmer, Chem. Soc. Rev., 2009, 38, 2823

_________________________

Last updated April 12, 2012 by Crima Pogge, all rights reserved.